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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been noticed in lots of tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was uncovered to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle regulated histone gene expression and performs a crucial role in endorsing cell-cycle development in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been explained. Two extra splice variants were being documented but comprehensive nucleotide sequence info isn't nonetheless obtainable.
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Blocking peptides are peptides that bind especially into the target antibody and block antibody binding. These peptide commonly contains the epitope acknowledged because of the antibody.
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This tutorial outlines even further probable optimization of cellular and immunoassay parameters to make sure the best possible results are...
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This information outlines even further doable optimization of mobile and immunoassay parameters to guarantee the very best success are...
Antibodies sure to the blocking peptide now not bind to your epitope to the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, one can see which staining is unique.
Concentrate on Facts Cyclin E belongs on the hugely conserved cyclin household, whose associates are characterized by a dramatic periodicity in protein abundance in the cell cycle. Cyclins perform as regulators of CDK kinases. Unique cyclins show unique expression and degradation patterns which lead to the temporal coordination of every mitotic party. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates in the G1-S section boundary and is also degraded as cells progress via S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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